elisa method Search Results


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(A) The raw spectral emission from 200 - 300 nm of the filtered KrCl excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations. (B) The absorbance spectrum from 200 - 300 nm of <t>SARS-CoV-2</t> at ∼10 5 PFU/mL in cDMEM was measured for each of three biologically independent Tests for use in UV dose calculations. Expanded emission and absorbance spectra from 200 - 800 nm are shown in .
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(A) The raw spectral emission from 200 - 300 nm of the filtered KrCl excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations. (B) The absorbance spectrum from 200 - 300 nm of <t>SARS-CoV-2</t> at ∼10 5 PFU/mL in cDMEM was measured for each of three biologically independent Tests for use in UV dose calculations. Expanded emission and absorbance spectra from 200 - 800 nm are shown in .
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(A) The raw spectral emission from 200 - 300 nm of the filtered KrCl excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations. (B) The absorbance spectrum from 200 - 300 nm of <t>SARS-CoV-2</t> at ∼10 5 PFU/mL in cDMEM was measured for each of three biologically independent Tests for use in UV dose calculations. Expanded emission and absorbance spectra from 200 - 800 nm are shown in .
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(A) The raw spectral emission from 200 - 300 nm of the filtered KrCl excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations. (B) The absorbance spectrum from 200 - 300 nm of <t>SARS-CoV-2</t> at ∼10 5 PFU/mL in cDMEM was measured for each of three biologically independent Tests for use in UV dose calculations. Expanded emission and absorbance spectra from 200 - 800 nm are shown in .
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(A) The raw spectral emission from 200 - 300 nm of the filtered KrCl excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations. (B) The absorbance spectrum from 200 - 300 nm of <t>SARS-CoV-2</t> at ∼10 5 PFU/mL in cDMEM was measured for each of three biologically independent Tests for use in UV dose calculations. Expanded emission and absorbance spectra from 200 - 800 nm are shown in .
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(A) The raw spectral emission from 200 - 300 nm of the filtered KrCl excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations. (B) The absorbance spectrum from 200 - 300 nm of <t>SARS-CoV-2</t> at ∼10 5 PFU/mL in cDMEM was measured for each of three biologically independent Tests for use in UV dose calculations. Expanded emission and absorbance spectra from 200 - 800 nm are shown in .
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(A) The raw spectral emission from 200 - 300 nm of the filtered KrCl excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations. (B) The absorbance spectrum from 200 - 300 nm of <t>SARS-CoV-2</t> at ∼10 5 PFU/mL in cDMEM was measured for each of three biologically independent Tests for use in UV dose calculations. Expanded emission and absorbance spectra from 200 - 800 nm are shown in .
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(A) The raw spectral emission from 200 - 300 nm of the filtered KrCl excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations. (B) The absorbance spectrum from 200 - 300 nm of <t>SARS-CoV-2</t> at ∼10 5 PFU/mL in cDMEM was measured for each of three biologically independent Tests for use in UV dose calculations. Expanded emission and absorbance spectra from 200 - 800 nm are shown in .
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Abbott Laboratories elisa method axsym/core/hbe
(A) The raw spectral emission from 200 - 300 nm of the filtered KrCl excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations. (B) The absorbance spectrum from 200 - 300 nm of <t>SARS-CoV-2</t> at ∼10 5 PFU/mL in cDMEM was measured for each of three biologically independent Tests for use in UV dose calculations. Expanded emission and absorbance spectra from 200 - 800 nm are shown in .
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Pishtaz Teb commercial elisa kits pishtaz teb zaman diagnostics
(A) The raw spectral emission from 200 - 300 nm of the filtered KrCl excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations. (B) The absorbance spectrum from 200 - 300 nm of <t>SARS-CoV-2</t> at ∼10 5 PFU/mL in cDMEM was measured for each of three biologically independent Tests for use in UV dose calculations. Expanded emission and absorbance spectra from 200 - 800 nm are shown in .
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(A) The raw spectral emission from 200 - 300 nm of the filtered KrCl excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations. (B) The absorbance spectrum from 200 - 300 nm of SARS-CoV-2 at ∼10 5 PFU/mL in cDMEM was measured for each of three biologically independent Tests for use in UV dose calculations. Expanded emission and absorbance spectra from 200 - 800 nm are shown in .

Journal: medRxiv

Article Title: SARS-CoV-2 disinfection in aqueous solution by UV 222 from a krypton chlorine excilamp

doi: 10.1101/2021.02.19.21252101

Figure Lengend Snippet: (A) The raw spectral emission from 200 - 300 nm of the filtered KrCl excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations. (B) The absorbance spectrum from 200 - 300 nm of SARS-CoV-2 at ∼10 5 PFU/mL in cDMEM was measured for each of three biologically independent Tests for use in UV dose calculations. Expanded emission and absorbance spectra from 200 - 800 nm are shown in .

Article Snippet: The concentration of N protein in outgrowth assay supernatants was determined using the SARS-CoV-2 Antigen Quantitative Assay Kit (ELISA) method (ADS Biotec).

Techniques:

(A) SARS-CoV-2 titers measured by plaque assay 3 days after sample exposure to each UV 222 dose (dark circles) were fit with an exponential model starting at the mean initial (0 mJ/cm ) viral titer of 6.51×10 4 PFU/mL through responses up to and including 8 mJ/cm where PFU/mL first dropped below the assay detection limit (DL) of 2 PFU/mL (hollow circles). Error bars represent standard deviation of at least two technical replicates. (B) SARS-CoV-2 log 10 reductions (LR) of viral titers after exposure to each UV 222 dose (dark circles) were fit with a linear model forced through the origin at 0 mJ/cm through responses up to and including 8 mJ/cm where LR first exceeded the DL of 4.51 logs (hollow circles).

Journal: medRxiv

Article Title: SARS-CoV-2 disinfection in aqueous solution by UV 222 from a krypton chlorine excilamp

doi: 10.1101/2021.02.19.21252101

Figure Lengend Snippet: (A) SARS-CoV-2 titers measured by plaque assay 3 days after sample exposure to each UV 222 dose (dark circles) were fit with an exponential model starting at the mean initial (0 mJ/cm ) viral titer of 6.51×10 4 PFU/mL through responses up to and including 8 mJ/cm where PFU/mL first dropped below the assay detection limit (DL) of 2 PFU/mL (hollow circles). Error bars represent standard deviation of at least two technical replicates. (B) SARS-CoV-2 log 10 reductions (LR) of viral titers after exposure to each UV 222 dose (dark circles) were fit with a linear model forced through the origin at 0 mJ/cm through responses up to and including 8 mJ/cm where LR first exceeded the DL of 4.51 logs (hollow circles).

Article Snippet: The concentration of N protein in outgrowth assay supernatants was determined using the SARS-CoV-2 Antigen Quantitative Assay Kit (ELISA) method (ADS Biotec).

Techniques: Plaque Assay, Standard Deviation

(A) SARS-CoV-2 N gene damage immediately after UV treatment (Day 0) and after incubation of samples with host cells (Day 3) expressed as log 10 reduction of N1 (short amplicon) copies/µL in qPCR reactions. (B) SARS-CoV-2 N gene damage immediately after UV treatment (Day 0) expressed as log 10 reduction of N1-2 (long amplicon) copies/µL in qPCR reactions. (C) SARS-CoV-2 N protein concentration measured by ELISA expressed as log 10 reduction of N protein concentration (pg/mL) in samples immediately after UV treatment (Day 0) and after incubation of samples with host cells (Day 3). SARS-CoV-2 log 10 reductions of the N1 amplicon, N1-2 amplicon, or N protein versus UV 222 dose were fit with a linear model forced through the origin at 0 mJ/cm through responses up to and including 20 mJ/cm indicated by filled circles. Points not included in models are indicated by hollow circles.

Journal: medRxiv

Article Title: SARS-CoV-2 disinfection in aqueous solution by UV 222 from a krypton chlorine excilamp

doi: 10.1101/2021.02.19.21252101

Figure Lengend Snippet: (A) SARS-CoV-2 N gene damage immediately after UV treatment (Day 0) and after incubation of samples with host cells (Day 3) expressed as log 10 reduction of N1 (short amplicon) copies/µL in qPCR reactions. (B) SARS-CoV-2 N gene damage immediately after UV treatment (Day 0) expressed as log 10 reduction of N1-2 (long amplicon) copies/µL in qPCR reactions. (C) SARS-CoV-2 N protein concentration measured by ELISA expressed as log 10 reduction of N protein concentration (pg/mL) in samples immediately after UV treatment (Day 0) and after incubation of samples with host cells (Day 3). SARS-CoV-2 log 10 reductions of the N1 amplicon, N1-2 amplicon, or N protein versus UV 222 dose were fit with a linear model forced through the origin at 0 mJ/cm through responses up to and including 20 mJ/cm indicated by filled circles. Points not included in models are indicated by hollow circles.

Article Snippet: The concentration of N protein in outgrowth assay supernatants was determined using the SARS-CoV-2 Antigen Quantitative Assay Kit (ELISA) method (ADS Biotec).

Techniques: Incubation, Amplification, Protein Concentration, Enzyme-linked Immunosorbent Assay

(A) The raw spectral emission from 200 - 300 nm of the filtered excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations and plotted on log scale to show orders of magnitude less but non-zero emission at filtered wavelengths > 240 nm. (B) The absorbance spectrum from 200 - 300 nm of SARS-CoV-2 at ∼10 5 PFU/mL in cDMEM was measured for each of three Tests for use in UV dose calculations.

Journal: medRxiv

Article Title: SARS-CoV-2 disinfection in aqueous solution by UV 222 from a krypton chlorine excilamp

doi: 10.1101/2021.02.19.21252101

Figure Lengend Snippet: (A) The raw spectral emission from 200 - 300 nm of the filtered excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations and plotted on log scale to show orders of magnitude less but non-zero emission at filtered wavelengths > 240 nm. (B) The absorbance spectrum from 200 - 300 nm of SARS-CoV-2 at ∼10 5 PFU/mL in cDMEM was measured for each of three Tests for use in UV dose calculations.

Article Snippet: The concentration of N protein in outgrowth assay supernatants was determined using the SARS-CoV-2 Antigen Quantitative Assay Kit (ELISA) method (ADS Biotec).

Techniques: